2 × Phanta® Max Master Mix (Dye Plus)

Phanta® Max has added unique elongation factors, specificity promoting factors and plateau uninhibiting factors, which greatly improves the ability of long fragment amplification, amplification specificity and amplification yield. Using simple templates such as λDNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta® Max can effectively amplify fragments up to 20 kb; using cDNA templates, Phanta® Max It can effectively amplify fragments up to 10 kb. Its amplification mismatch rate is 1/53 of that of ordinary Taq polymerase and 1/6 of that of Pfu polymerase. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max contains two monoclonal antibodies that can inhibit its 5’ → 3’ polymerase activity and 3’ → 5’ exonuclease activity at room temperature, enabling highly specific hot-start PCR. This product contains Phanta® Max Super-Fidelity DNA Polymerase, dNTP and an optimized buffer system. It only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added to the system allows the 2 × Master mix to maintain stable activity after repeated freezing and thawing; the system contains an electrophoresis indicator, which can be directly spotted for electrophoresis after the PCR reaction is completed. The enzyme has 5'→ 3'polymerase activity and 3'→ 5'exonuclease activity, and the amplified product is blunt-ended, suitable for ClonExpress® and topological cloning kits (C112/C113/C115/C601).Application: Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches Faster amplification: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments Longer amplification: the effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of genome can reach 20 kb, and the effective amplification length of cDNA can reach 10 kb. Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples Simple operation: Dye premix, minimize experimental steps