2 × Taq Plus Master Mix II (Dye Plus)

This product contains Taq Plus DNA Polymerase, dNTP and optimized buffer system, suitable for high-yield PCR reactions. Compared with ordinary PCR, it has higher fidelity, stronger amplification performance and yield. It can be used for PCR amplification within 10 kb using genome as template and PCR amplification within 15 kb using plasmid and λDNA as template . The pre-prepared 2×Taq Plus Master Mix II only needs to add primers and templates to perform amplification when used in PCR reactions, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added to the amplification system allows 2 × Taq Plus Master Mix II to maintain stable activity after repeated freezing and thawing. This product contains blue double dyes, which can be directly electrophoresed after the reaction, which is convenient to use. The PCR product has an A at the 3'end, which can be cloned directly into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113/C114).Application: Strong amplification ability: For most templates and primers, the yield is significantly improved; High visibility: The color of the sample will be clearer when the PCR reaction is completed and the sample is added by agarose gel electrophoresis; Convenient operation: amplification can be carried out by adding primers and templates, no operation on ice is required, and the product containing dye can be directly subjected to electrophoresis; Good stability: 50 times of repeated freezing and thawing, no significant decrease in activity