2 × Vazyme LAmp Master Mix (Dye Plus)

Vazyme LAmp® DNA Polymerase is a mixed enzyme composed of Taq DNA Polymerase and a protein containing 3’→5’ exonuclease activity (proofreading activity). The fidelity is Taq DNA 6 times that of Polymerase. With a specially optimized buffer system, Vazyme LAmp® DNA Polymerase is very suitable for the amplification of long fragments. It can amplify fragments up to 21kb from the genome, and has high amplification efficiency for templates of different sources and different lengths. This product contains Vazyme LAmp® DNAPolymerase, dNTP and an optimized buffer system. It can be amplified by adding primers and templates, which reduces pipetting operations and improves throughput and results reproducibility. The protective agent added to the system allows Master Mix to maintain stable activity after repeated freezing and thawing. This product also provides a version containing electrophoresis buffer and dye, which can be directly electrophoresed after the reaction, which is convenient to use. The PCR product has an A at the 3'end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113). Application: Efficient amplification of genome and cDNA fragments >20 kb; Very high amplification sensitivity and strong tolerance to low purity templates; Broad GC adaptability; Provide ready-to-use premix to minimize experimental steps