M-MLV(H-) Reverse Transcriptase

Wild-type M-MLV (Moloney Murine Leukemia Virus) has the following activities: RNA-dependent DNA polymerase activity; DNA-dependent DNA polymerase activity; RNase H activity. Because RNase H activity can catalyze the degradation of RNA in the DNA/RNA hybrid strand, the template RNA may be degraded in the cDNA one-strand synthesis reaction. M-MLV (H-) Reverse Transcriptase is an M-MLV mutant with loss of RNase H activity obtained by site-directed mutagenesis. Compared with the common mutants obtained by deleting the RNase H domain, this product retains the complete protein structure, so it has the same polymerase activity as the wild type, and can be used for longer cDNA synthesis and full-length cDNA library Construction, etc. It also has strand displacement activity and can be used in experiments such as 5'RACE.Application: Point mutations eliminate RNase H activity to obtain long cDNA products; Stable and reliable reverse transcription performance for RNA templates above 100 ng; Synthetic fragment ≤5 kb; Can be used for experiments such as 5’-RACE reaction and cDNA library construction