HiScript II 1st Strand cDNA Synthesis Kit

HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation of HiScript® Reverse Transcriptase, HiScript® II has further greatly improved thermal stability and is very suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HiScript® II adds multiple point mutations, which further enhances template affinity and progress, and has higher tolerance to common reverse transcription inhibitors, which greatly improves the ability to synthesize full-length cDNA. HiScript® II 1st Strand cDNA Synthesis Kit is a first-strand cDNA synthesis kit based on HiScript® II Reverse Transcriptase. It contains all the components needed to synthesize high-quality first-strand cDNA. The product is suitable for subsequent PCR, qPCR and other experiments. 2 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo (dT)23VN included in the kit has a stronger anchoring ability for Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo (dT)23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can not only synthesize full-length cDNA (up to 20 kb) for cloning, but also efficiently synthesize cDNA with uniform reverse transcription efficiency for each position of qPCR.Application: Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA; A wide range of template starting amount, from 1 pg-5 μg total RNA, and can amplify fragments up to 15 kb or more; Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis; Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.