HiScript II One Step RT-PCR Kit (Dye Plus)

HiScript® II One Step RT-PCR Kit (Dye Plus) is specially designed for end-point PCR detection using RNA as a template (such as RNA virus). PCR products can be directly loaded for electrophoresis, which greatly saves time and labor. Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which improves detection throughput and reduces the risk of contamination. Integrating the superior performance of HiScript® II Reverse Transcriptase and Champagne TaqTM plus DNA Polymerase, with an optimized buffer system, the detection sensitivity of HiScript® II One Step RT-PCR Kit (Dye Plus) can reach 1 pg total RNA and can be amplified Fragments up to 10 kb. The kit is provided in a convenient Master Mix format. 2 × One Step Mix (Dye Plus) contains optimized buffer system, dNTP and loading dye; One Step Enzyme Mix contains optimized ratio of HiScript® II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM plus DNA Polymerase.Application: Reverse transcription and PCR are performed in the same tube; The detection sensitivity can reach 0.1 pg total RNA; Amplify long fragments up to 10 kb or more; PCR products can be directly loaded for electrophoresis