HiScript II Q Select RT SuperMix for qPCR

HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Greatly improve thermal stability and cDNA synthesis efficiency. HiScript® II Q Select RT SuperMix for qPCR is suitable for two-step qRT-PCR detection. 5 × HiScript II Select qRT SuperMix contains Buffer, dNTP Mix, HiScript II Reverse Transcriptase and RNase inhibitor. Oligo (dT) can be selected as required 23VN, Random primers or gene-specific primers are used as reverse transcription primers. Oligo (dT)23VN has stronger anchoring ability for Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. 5 × HiScript® II Select qRT SuperMix will not freeze at -20℃, and the reaction can be performed quickly by adding template RNA and primers. The reverse transcription product is compatible with SYBR® Green and probe method qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for use in conjunction with high-performance gene expression analysis. Application: Based on the efficient HiScript ® II Reverse Transcriptase, the reverse transcription efficiency is higher; Different types of reverse transcription primers can be used flexibly for different experimental designs; Ready-to-use SuperMix premix is ​​easy to use and time-saving