miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) is a special kit suitable for stem-loop miRNA cDNA one-strand synthesis. It contains genomic DNA removal steps, which can quickly remove genomic DNA contamination at 42℃ for 2 min. The follow-up results are more reliable. The HiScript ® II Reverse Transcriptase on which the kit is based has extremely high thermal stability, coupled with an optimized buffer system, which is conducive to the generation of miRNA-specific reverse transcription products. For subsequent quantification of cDNA products, it is recommended to use our company's miRNA Universal SYBR ® qPCR Master Mix (Vazyme # MQ101) to obtain the best experimental results. At the same time, the design software for miRNA reverse transcription stem-loop primers and quantitative primers was launched, which is easy to operate and escort miRNA experiments.Application: Excellent linear relationship: It has a good linear relationship in a wide template interval, and can detect RNA templates as low as pg; Superior reaction system: Optimal Buffer composition and concentration, more suitable for reverse transcription of microRNA; Simple primer design: Provide matching primer design software to make primer design easier