HiScript II One Step qRT-PCR SYBR Green Kit

HiScript® II One Step qRT-PCR SYBR® Green Kit uses the SYBR® Green I chimeric fluorescence method and is designed for quantitative PCR detection using RNA as a template (such as RNA viruses). Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which greatly improves detection throughput and reduces the risk of contamination. Integrating the superior performance of HiScript® II Reverse Transcriptase and Champagne TaqTM DNA Polymerase, with an optimized buffer system, the detection sensitivity of the One Step qRT-PCR SYBR® Green Kit can reach 1 pg of total RNA. The enhancement factor added to the buffer can effectively reduce the formation of primer dimers and improve specificity. The kit is provided in a convenient Master Mix format. 2 × One Step SYBR® Green Mix contains optimized buffer system, dNTP, specific enhancement factor and SYBR® Green I fluorescent dye; One Step SYBR® Green Enzyme Mix contains optimized ratio of HiScript® II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM DNA PolymeraseApplication: Excellent specificity: special additive components can minimize the generation of primer dimers, and the quantitative results are more accurate; Ultra-high detection sensitivity: taking into account high amplification efficiency, it can detect as little as 1 pg of total RNA template