DNase I , RNase-free

DNase I (deoxyribonuclease I), that is, Deoxyribonuclease I, is an endodeoxyribonuclease that can digest single-stranded or double-stranded DNA. It recognizes and cleaves phosphodiester bonds to produce a 5'-end phosphate group Group, 3'-end hydroxyl monodeoxynucleotide or single-stranded or double-stranded oligodeoxynucleotide. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions Mg2+, Mn2+, etc. It exists in Mg2+ In the case of Mn2+, the enzyme can randomly recognize and cut any site on any strand of double-stranded DNA; and in the presence of Mn2+, it can recognize and cut almost the same site on both strands of DNA, resulting in blunt ends or There are sticky-end DNA fragments with 1-2 nucleotides protruding. This product has undergone strict quality control and is suitable for RNA extraction, in vitro transcription, DNA removal in RT-PCR experiments, DNase I footprinting, nick translatioin, DNA random fragment library construction and other molecular biology experiment. Application: High activity Very low RNase residue Good stability