Ribo-off® Globin & rRNA Depletion Kit (Human/Mouse/Rat)

Ribo-off Globin & rRNA Depletion Kit (Human/Mouse/Rat) is a kit for removing Globin mRNA and rRNA from total RNA in blood samples. This kit is compatible with 0.01 – 1 μg blood total RNA. The total RNA sample undergoes probe hybridization, RNase H digestion, DNase I digestion, etc. Finally, Globin mRNA and rRNA (including cytoplasmic rRNA and mitochondrial rRNA) are removed from total RNA, leaving other mRNA and non-coding RNA, which can be used for the analysis of non-coding RNA such as LncRNA. This kit is compatible with partially degraded RNA samples, and the obtained products are suitable for RNA library construction and other experiments.Application: Ribo-off Globin & rRNA Depletion Kit (Human/Mouse/Rat) is suitable for removing Globin mRNA (including HBA1/2, HBB, HBG1/2) and rRNA (including cytoplasmic 28S, 18S, 5.8S, 5S rRNA, and mitochondrial 16S, 12S rRNA) from 0.01 - 1 μg blood samples, leaving other mRNA and non-coding RNA. It is compatible with partially degraded RNA samples, and the products are suitable for RNA library construction and other experiments. The mRNA content in total RNA of different samples varies greatly. According to the downstream applications, the initial template amount of total RNA can be adjusted appropriately. The products can be used for library construction with VAHTS Universal V6 RNA-seq Library Prep Kit for lllumina (Vazyme NR604), VAHTS Universal V8 RNA-seq Library Prep Kit for lllumina (Vazyme NR605).