VAHTS HiFi Amplification Mix

VAHTSTM HiFi Amplification Mix is ​​a new high-fidelity PCR amplification master mix, suitable for PCR amplification of high-throughput sequencing libraries. The master mix is ​​based on VAHTSTM HiFi DNA Polymerase, which is a new generation of high-yield, high-fidelity DNA polymerase modified from Pfu DNA Polymerase. The sensitivity is greatly improved, and it has extremely high amplification efficiency and wide template adaptability. Expansion of output and plateau period. At the same time, its amplification mismatch rate is 1/52 of Taq DNA Polymerase and 1/6 of Pfu DNA Polymerase, ensuring the authenticity of library amplification. Coupled with a carefully optimized buffer system, VAHTSTM HiFi Amplification Mix can achieve low preference, wide template input range, high yield, and high stability amplification of high-throughput sequencing libraries. This product contains all the components required for PCR amplification, and contains library amplification primers for the Illumina sequencing platform. It is only necessary to add the library template to perform the reaction, and it is convenient to use; it contains a special protective agent and is stored for a long time or after repeated freezing and thawing. Can still maintain stable activity. The amplified product is blunt-ended and can be directly used for blunt-ended cloning.Application: Single-tube end repair, dA tailing reaction; End repair plus A takes less time, only 30 minutes; The reagents are in Mix form, compatible with automated library building equipment The template amount has a wider compatibility range, and can efficiently repair 100 pg-1 μg Input DNA; A wider range of sample types are compatible, suitable for cfDNA, FFPE and fragmented DNA