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Quote DNA polymerase I Klenow fragment
DNA polymerase I, the large fragment (Klenow fragment) is the protease hydrolysate of E. coli DNA polymerase I, with 5´→3´ DNA polymerase activity and 3´→5´ exonuclease activity, but 5 is missing ´→3´ Exonuclease activity. The Klenow fragment retains the high fidelity of the holoenzyme without degrading the 5´ end of DNA.Application: Simple and rapid isothermal reaction speeds up the preparation of plasmid samples for sequencing;
High fidelity, strong chain switching activity, suitable for whole genome amplification
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Reference:
N104-01
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