Phanta Uc Super-Fidelity DNA Polymerase for Library Amplification

Phanta® Uc Super-Fidelity DNA Polymerase is a new generation of high-fidelity DNA polymerase modified from Pfu DNA Polymerase, with extremely high amplification efficiency Rate and wide template adaptability. Compared with Pfu DNA Polymerase, Phanta® Uc Super-Fidelity DNA Polymerase has greatly improved its mobility. Even very complex templates can complete PCR reactions quickly and accurately. Its mismatch rate is 1/52 of Taq DNA Polymerase and 1/6 of Pfu DNA Polymerase, and it completely overcomes the inability of conventional high-fidelity enzymes to use dUTP as raw material for polymerization, the inability to use amplification primers containing dUTP, and the inability to use DNA containing dUTP is used as an amplification template and many other disadvantages. With a carefully optimized reaction buffer for library amplification, Phanta® Uc Super-Fidelity DNA Polymerase for Library Amplification can achieve high-throughput measurement The low preference, high efficiency and high stability amplification of sequence library. The amplified product is blunt-ended and can be directly used for blunt-ended cloning. Application: DNA or RNA 5'end is phosphorylated for ligation reaction; End labeling of DNA or RNA for use as probes and DNA sequencing