VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina

VAHTSTM Total RNA-seq (H/M/R) Library Prep Kit for Illumina® is a special kit for chain-specific transcriptome library construction targeted for the Illumina high-throughput sequencing platform. This kit is suitable for the amount of initial template The total RNA of human, mouse, and rat is 0.1-1 μg. The difference from conventional transcriptome library is that this kit can combine rRNA (including cytoplasm 28S, 18S, 5S rRNA and mitochondrial 16S, 12S, 5.8S rRNA). ) Is removed from total RNA, leaving mRNA and other non-coding RNAs Used for the analysis of non-coding RNA such as LncRNA; degraded RNA samples can also be used for library construction with this kit. When the second-strand cDNA is synthesized in this kit, dUTP is added to label it, and the labeled second-strand is digested with UDG enzyme before PCR, so the sequencing information comes from the first-strand cDNA, which ensures the strand directionality of RNA Analyze the sequencing data to determine whether the transcript is from the sense or antisense DNA strand. The kit includes three independent packages of NR4, NR5 and NR6, which contain the library construction process All necessary enzymes, buffers, and all components have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent. Adapters and DNA and RNA purification magnetic beads need to be prepared separately. Adapters can be selected from VAHTSTM RNA Adapters (Vazyme#N803, Vazyme #N804 or Vazyme #N809, Vazyme #N810, Vazyme #N811, Vazyme#N812). For details on adapter selection, please refer to Common Problems and Solutions 6 (Page17); DNA purification magnetic Beads can choose VAHTSTM DNA Clean Beads (Vazyme #N411) or Agencourt® AMPure® XP reagent (Beckman #A63880,#A63881, #A63882); RNA purification magnetic beads can choose VAHTSTM RNA Clean Beads (Vazyme #N412) or Agencourt® RNAClean ® XP Beads (Beckman #A63987). Application: Fast: The minimalist operation process reduces the time for building a database by 2 h Compatibility: Compatible with a variety of RNA enrichment modules, with any combination General: Flexible and free choice of chain-specific or non-chain-specific libraries