VAHTS Small RNA Library Prep Kit for Illumina

VAHTSTM Small RNA Library Prep Kit for Illumina® is a special kit for constructing small RNA libraries targeted for the Illumina® high-throughput sequencing platform. This kit is suitable for the total RNA of animals and plants with a template starting amount of 0.1-1 μg, as well as the separation and purification of small RNA. Connect the 3'and 5'ends of small RNA with universal adapters, and then go through the steps of reverse transcription, PCR amplification, and purification by PAGE gel or magnetic beads to finally obtain a sequencing library suitable for the Illumina® platform. The kit contains all the enzymes and buffers required for library construction. All components have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.Application: VAHTS Small RNA Library Prep Kit for Illumina® is applicable to input template with 0.1-1 ug of animal or plant total RNA with good integrity (RIN ≥ 7) or purified small RNA. The content of small RNA in total RNA of different samples varied greatly. If the input of total RNA is too low, it may lead to the unsuccessful library construction. The proportion of rRNA or other RNA in the library will increase if incomplete or degraded (RIN < 7) total RNA are used. It is recommended to use Agilent RNA 6000 Pico Kit to control the quality of the input total RNA and to avoid using RNA samples with RIN < 7.