VAHTS Universal V6 RNA-Seq Library Prep Kit for MGI

VAHTS® Universal V6 RNA-seq Library Prep Kit for MGI is a special kit for transcriptome library construction targeted for the MGI high-throughput sequencing platform. The kit contains two types of cDNA two-strand synthesis buffers, which can be selected for general transcriptome or strand-specific transcription according to needs. The kit combines two-strand synthesis, end repair, and dA-Tailing into one step. No purification steps are required in the middle, which greatly simplifies the operation process and shortens the time for library construction. The optimized reaction system improves the efficiency of library conversion, is compatible with lower initial input, and has uniform coverage for different initial amounts of RNA. This kit uses magnetic bead sorting to quickly obtain a library of a specific length, which can meet the individual needs of different experiments. All enzymes and buffers included in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.Application: VAHTS Universal V6 RNA-seq Library Prep Kit for MGI is suitable for RNA library construction of RNA that have been enriched by Poly(A) (for RNA with good integrity from eukaryotes such as animals, plants and fungi) or rRNA depletion. As the content of mRNA in total RNA of different samples varies greatly, enough total RNA need to be inputted to make sure the sufficient mRNA for library construction. The amount of input-RNA is related to the mRNA enrichment module: VAHTS mRNA Capture Beads (Vazyme N401): 0.05μg - 4 μg; Ribo-off rRNA Depletion Kit (H/R/M) (Vazyme N406): 0.05μg - 1 μg; Ribo-off rRNA Depletion Kit (Bacteria) (Vazyme N407): 1 μg - 5 μg; Ribo-off rRNA Depletion Kit (Plant) (Vazyme N409): 1 μg - 5 μg. It is recommended to use an Agilent 2100 Bioanalyzer to analyze the integrity of total RNA. mRNA enrichment using VAHTS® mRNA Capture Beads (Vazyme N401) needs high quality RNA samples (RIN ≥ 7). Degraded total RNA used for library construction will lead to 3’ bias in RNA-seq. For RNA samples with RIN value < 7, rRNA removal can be performed using the Ribo-off method (Vazyme N406/407/409). Main fields of RNA-related analysis: gene expression analysis, single nucleotide variation calling, alternative splicing detection, gene fusion detection or target transcriptome analysis