Nickel Coated Plates, Clear, 12×8-Well Strips, White Frame

Porath first discovered in 1975 that transition state metal ions immobilized on a substrate can achieve both specific binding effects through coordination with residues such as histidine, cysteine, tryptophan on the protein surface. The metal chelate affinity technique has become a powerful tool for the research and development of biopharmaceuticals because of its high selectivity, high adsorption capacity, mild conditions, high selectivity, excellent separation efficiency, reproducibility and versatility. The Nickel coated plate, a solid-state support for the purification / enrichment / capture of histidine (His) - tagged proteins through the chelation immobilization of Ni2 + combined with triacetic acid nitrate (NTA), are one of the most commonly used metal chelate affinity techniques.