Triton X-100 (25%)

Non ionic detergents like Triton X-1 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from .1- .5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background.Triton X-1 (polyethyleneglycolp (1,1,3,3 tetramethylbutyl) phenylether ,octylphenolethoxylate, polyoxyethylene octyl phenyl ether,4-octylphenol polyethoxylate,Mono 3, TX-1, t-octylphenoxypolyethoxyethanol, Octoxynol-9it lower surface tension of water. Triton X-1 is also used for recovery of membrane components under mild non-denaturing conditions.