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Z-Leu-Leu-Glu-AMC [Z-LLE-AMC]

Z-Leu-Leu-Glu-AMC [Z-LLE-AMC]

Z-LLE-AMC is a fluorogenic peptide substrate for measuring caspase-like activity of the proteasome. Hydrolysis of this substrate by the beta1-subunit of the 20S proteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm. This substrate is specific to the constitutive proteasome and is not hydrolyzed efficiently by the immunoproteasome. 20S proteasome enzyme requires activation with 0.035% SDS in the assay buffer.

Z-Leu-Leu-Glu-AMC [Z-LLE-AMC]

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