BioStab Peroxidase Conjugate Stabilizer

Horseradish peroxidase (HRP) is an enzyme commonly used for immunodetection. BioStab Peroxidase Conjugate Stabilizer extends the lifetime of HRP-antibody conjugates and increases the signal intensity, allowing much more consistent results over extended periods of time compared to daily prepared dilutions. The mode of action of BioStab HRP Stabilizer comprises both stabilization of the antibody and stabilization of the enzymatically active centre of HRP. E.g. after 4 weeks at 37 ° C at a ready-to use concentration of 100 ng/ml residual activity comprises more than 97 % of the initial enzyme activity as well as the antibody binding properties. Compared to immediately made dilutions in an unspecific dilution buffer, 10 to up to 30 % higher activities are observed in BioStab HRP Stabilizer, indicating a stabilization of the solution compared to inhibition of the enzyme in normal buffers. BioStab Peroxidase Conjugate Stabilizer has only minimal effects on the enzymatic reaction.Thus HRP conjugates can be used at ready-touse concentrations in BioStab Peroxidase Conjugate Stabilizer for a minimum of 6 months at room temperature or 1 year at 4°C.