AgriBlock | Non mammalian Protein Membrane Blocker

Agrisera Non Mammalian Protein Membrane Blocker is a blot friendly formulation for optimal blocking effect.Featuresready to use, no mammalian protein includedstable for a minimum of 2 yearscontains antimicrobial agents (Bromidox, 0.08%) to prevent bacterial growthRecommendation for use:Following protein transfer to PVDF or nitrocellulose membrane, block the membrane with AgriBlock Non mammalian Protein Membrane Blocker, equilibrated to room temperature before use, for 1 hour at room temperature or overnight at 4ºC.Wash the membrane with TBST or PSBT three times per wash for 5 minutes, each with agitation on a shaker at room temperature.Perform further steps following western blot protocol for a given primary antibody:Add primary antibody of choice, at the appropriate optimized dilution using the recommended antibody diluent and incubate either 1h at room temperature or overnight at 4°C with agitation on a shaker.Wash the membrane with TBST of PBST, according to your protocol, with agitation on a shaker at room temperature.Add a matching secondary antibody at the appropriate optimized dilution and incubate for 1 hour with agitation on a shaker at room temperature.Wash the membrane with TBST of PBST, according to your protocol, with agitation on a shaker at room temperature.Proceed with visualization of reaction, depending upon the system which is applied, e.g. chemiluminescent (HRP-conjugated antibodies), chromogenic (ALP-conjugated antibodies) or fluorescent (for example DyLight®).