Agrisera BCIP/NBT Plus ALP Substrate

Agrisera BCIP/NBT Plus ALP Substrate is designed to localize ALP labeled probes on immunohistochemical , in situ hybridization and blotting techniques. When the BCIP/NBT substrate solution is added to a blotting system containing ALP labeled probes the phosphate group of BCIP is hydrolyzed by ALP. This results in the formation of reducing components that reacts with NBT, converting it to insoluble purple NBT formazan visible on the blotting membrane.Agrisera BCIP/NBT ALP Substrate contains BCIP (0.692 mMol L-1) and NBT (0.734 mMol L-1) in a 2-amino-2-methyl-1-propanol (AMP) buffer at a pH of 9,8 with added non-toxic stabilizers.Features:Ready to use, single component BCIP/NBT reagentNo to little backgroundContains non-toxic stabilizers