Effect Diluent Kit, Animal-free
They can be used for the dilution of serum, plasma, blood, stool or urine samples, as well as the dilution of primary and secondary antibodies. Effect Diluents, Animal-free efficiently minimize matrix effects, cross-reactions and unspecific binding in immunoassays like ELISA, Western blotting, Immunohistochemistry, protein arrays and immuno-PCR.
The Effect Diluents, Animal-free are used alternatively to the standard sample or antibody dilution buffers:
- In ELISA for the dilution of specimen and detection antibodies.
- In Western Blotting for the dilution of primary and secondary antibodies.
- In Protein arrays for the dilution of specimen and detection antibodies.
- In immuno-PCR as a washing buffer.
Three versions of the diluent are offered: Low, Medium and High for optimal discrimination between specific and unspecific reaction and for minimizing strong interference effects e.g., by RF (rheumatoid factors), HAMAs (human-a-mouse Abs) or by endogenous components that bind and mask the analyte.