Effect Diluent Medium, Animal-free

Effect Diluents, Animal-free are effective buffers free of any animal components.
They can be used for the dilution of serum, plasma, blood, stool or urine samples, as well as the dilution of primary and secondary antibodies. Effect Diluents, Animal-free efficiently minimize matrix effects, cross-reactions and unspecific binding in immunoassays like ELISA, Western blotting, Immunohistochemistry, protein arrays and immuno-PCR.

The Effect Diluents, Animal-free are used alternatively to the standard sample or antibody dilution buffers:

• In ELISA for the dilution of specimen and detection antibodies.
• In Western Blotting for the dilution of primary and secondary antibodies.
• In Protein arrays for the dilution of specimen and detection antibodies.
• In immuno-PCR as a washing buffer.

 Three versions of the diluent are offered: Low, Medium and High for optimal discrimination between specific and unspecific reaction and for minimizing strong interference effects e.g., by RF (rheumatoid factors), HAMAs (human-a-mouse Abs) or by endogenous components that bind and mask the analyte.