CheKine™ Aspartate Aminotransferase (AST/GOT) Activity Colorimetric Assay Kit

The kit provides a simple method for detecting AST/GOT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, Aspartate aminotransferase (AST) catalyzes the transamination reaction of α-ketoglutarate and L-aspartic acid at 37°C and pH 7.4 to produce glutamic acid and pyruvate; Pyruvate can interact with 2,4- Dinitrophenylhydrazine to produce pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm can reflect AST/GOT activity.