CheKine™ Total Iron-Binding Capacity (TIBC ) Assay Kit (Colorimetric)

CheKine™ Total Iron-Binding Capacity (TIBC ) Assay Kit (Colorimetric) provides a simple, sensitive, rapid TIBC detection method, suitable for detecting serum samples. The principle is that under alkaline conditions, serum transferrin can be bonded to Fe3+, and the remaining unbound Fe3+ can be reduced to Fe2+, the bonded Fe3+ could be released after acidification ,and then be reduced into Fe2+ . A purple compound could be produced by the reaction between Fe2+ and philo oxazine ,and this purple compound has a special wave at 562 nm. Respectively reading the OD562nm values A1 and A2 , and A1 is based on the unbound Fe3+ , A2 is based on the bounded Fe3+ . And the concentration of TIBC is proportionate to the difference between A2 and A1.