20S Immunoproteasome Assay Kit

South Bay Bio's kit is designed to test for specific activity of 20S immunoproteasome. The kit provides purified 20S immunoproteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC) and Caspase-like activity of the immunoproteasome subunits beta1i/ PSMB9 (PAL-AMC) and beta5i/PSMB8 (ANW-AMC). Additionally, the compound, ONX-0914, which can be used to inhibit specifically the subunit beta5i/LMP7 20S immunoproteasome is included. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission= 445nm; allowing for a real-time read out of 20S immunoproteasome specific activity.