Dual Luciferase Reporter Assay Kit

The Dual Luciferase Reporter Assay Kit is used to detect the fluorescence intensity of the Luciferin substrate after the reporter plasmid is transfected into the cells, so as to reflect the expression of luciferase and achieve the purpose of detecting gene regulation. First, use luciferin as a substrate to detect Firefly luciferase, and then use coelenterazine as a substrate to detect Renilla luciferase (Renilla luciferase) to achieve dual luciferase reporter genes Detection. The dual luciferase detection kit detects the activity of firefly luciferase to evaluate the effect of regulatory elements induced by stimuli; Renilla luciferase is used as an internal reference for correcting transfection efficiency to eliminate differences in cell number and transfection efficiency between wells To ensure reliable results.Application: The fluorescent signal is strong; Wide detection range; high sensitivity