Genetic screening libraries using CRISPR are a widely used technique with which you can identify genetic targets that drive biological responses in your cell lines or model systems of interest.
- Identify essential genes for growth and proliferation.
- Find genes responsible for resistance or sensitivity to compounds or treatments.
- Look for lethal synthetic interactions with known genetic lesions.
- Elucidate the mechanism of action or target pathway for a compound or drug
For this reason, Cellecta creates personalized CRISPR libraries for both Knock-Out and inactivation or activation of certain genes, using sgRNA and shRNA combined with Cas9 and Cas9 modified for activation or inactivation.
These diverse gene targeting libraries are ready to be introduced by lentiviral vectors, either already packaged or just the plasmids.