Methyldetect is a Danish company that serves the needs of researchers by providing fast, sensitive and quantitative DNA methylation assays that can be used on widely available laboratory equipment. Methyldetect provides Methylation Sensitive High Resolution Melting (MS-HRM) technology for researchers in the field of epigenetics.
The number of cytosines (C) in a PCR product, amplified from a sodium bisulfite modified template, depends on the methylation status of cytosines in the un-modified template. Sodium bisulfite preserves the methylated cytosines and modifies non-methylated cytosines into uracil, which subsequently is substituted with thymine during PCR. Thus, if the locus is methylated, a C rich PCR product is obtained after PCR amplification, and a T rich PCR product if the locus was non-methylated. These PCR products will display different melting profiles when subjected to Methylation-Sensitive High-Resolution Melting (MS-HRM) analyses.
The PCR product amplified from the non-methylated version of a specific locus will have relatively low melting temperature and melts earlier in the temperature gradient (red curve) than the PCR product amplified from the methylated version of the same locus, which will melt at relatively higher temperature (blue curve).
In summary, MS-HRM is a PCR methylation detection method, dependant on bisulfite modification, and enables cost effective, fast, and highly sensitive assessment of locus specific methylation status.
You can consult all the genes present in the current catalog here.
On the other hand, if your gene of interest is not in our catalog, we will develop the assay for you.
Both to request a different gene and to request more information or a quote, you can do so in the following form:
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