The AlphaThera mission is to make antibody labeling as simple and consistent as possible, giving scientists confidence in their bioconjugation reactions.
oYo-Link® site-specific antibody labeling technology and enabling a variety of applications including high-sensitivity ELISAs, flow cytometry, single-cell analysis, and more.
oYo-Link reagents consist of low molecular weight (~8 kDa), high-affinity antibody-binding domains that possess a photo-crosslinker within their Fc-binding site. Upon illumination with non-damaging Black-light, oYo-Link forms a covalent bond with the antibody. This procedure is referred to as Light-Activated Site-Specific Conjugation (LASIC). Any label that is attached to oYo-Link will be covalently attached to the desired antibody.
The site-specific nature of oYo-Link eliminates the shortcomings that plague conventional protein labeling techniques, such as heterogeneous products, reduced antibody functionality, and batch-to-batch variability. The high affinity, site-specific interaction between oYo-Link and the antibody allows labeling to be performed in all common storage buffers, including those containing Tris and/or BSA. Therefore, there is no need to desalt or purify your sample prior to labeling.
You can label as little as 1 µg of antibody at a time and work with antibody concentrations as low as 50 µg/mL. Therefore, you don’t need to concentrate or centrifuge your sample prior to labeling. In addition, if you don’t use all of your oYo-Link in your reaction, you can store the rest for later use.
The oYo-Link Antibody Labeling System can be used to site-specifically and covalently label the Fc region of almost any “off-the-shelf” antibody with biotin, drugs, click chemistry tags (e.g. azide, DBCO), enzymes (e.g. MNase), HyperBright™ fluorescent labels, oligonucleotides, HRP and more.
If you need more information or want a quote, fill in the following form:
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