Alithea Genomics

The BRB-seq library preparation protocol makes it possible for the first time to perform RNA-seq on a large number of RNA or blood samples in a fast, robust and cost-effective way.

BRB-seq

As opposed to standard RNA-seq – where all samples need to be processed individually throughout the library preparation workflow – with BRB-seq all samples can be pooled and processed in one single tube right after the first reverse transcription reaction.

Each kit contains unique BRB-seq barcodes that are designed to “tag” in the a sample-specific manner all the mRNA molecules of a given sample.

Once all the samples have been barcoded, they can be immediately pooled together in one tube, which drastically reduces costs and manual operations of the downstream steps and, as a consequence, of the overall workflow.

There are several kit formats, including processing different sample types, such as the MERCURIUS Blood BRB-seq kit where in addition to the benefits of standard BRB-seq, such as throughput and cost-effectiveness, they are combined with an integrated strategy of globin depletion. More information here.

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