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Home Our suppliers

Cube biotech

Cube Biotech

The Cube Biotech team serves the biotech and pharmaceutical community with its expertise on expression, purification, stabilization and functional/structural characterization of proteins. Their projects focus on the pharmaceutically relevant class of membrane proteins. Both products and services are offered. Both products and services are offered.

Cube offers a wide range of products for protein affinity chromatography and protein stabilization that are of high quality and manufactured by Cube Biotech. The product offering is complemented by reagents such as detergents, cell-free expression lysates, nanodisc scaffold proteins, and patented membrane protein crystallization plates. Some particularly relevant membrane proteins (GPCRs) are available as fully characterized preparations. Their services cover the expression, purification, stabilization and crystallization of soluble and membrane proteins for applications like cryo electron microscopy, antibody generation, crystallization and assays.

Products

  • Nanodiscs: They stabilize membrane proteins. This innovative technology mimics the amphipathic environment of a lipid bilayer and thus, maintains the structure of the membrane protein in a physiologically relevant state
  • Protein Purification Products : Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. There are multiple ways to gain this homogeneity, but mostly affinity tags are used together with a matching purification resin.
  • Protein Purification Kits : Time is of essence inside a laboratory. Following this tenet we offer our PureCube agarose resins in practical Kits for your convenience. Equipped with columns, all necessary buffers and of course our hight quality resins.
  • Cell-Free Lysates : For increased convenience and higher protein yields (up to 6 mg/ml GFP), the HiYield-T7 lysate was developed. It already includes the T7 RNA polymerase enzyme.
  • Cubic Phase Crystallization : Lipidic cubic phase crystallization is the most popular method for membrane protein structure determination.
  • Pure & Active Membrane Proteins : GPCRs are both available solubilized in detergent, or reconstituted into nanodiscs for stabilization.
  • Plastic & Accessories
  • Ultrapure Detergents : Detergents are the most commonly used substances for membrane protein stabilization. Before you use a specific detergent for a new membrane protein you must screen for a matching one. For this purpose we offer our Detergent Screening Sets.
PRODUCTS

Services

Cube offers small to mid-scale, non-GMP protein services for a broad range of applications. Individual work packages, made up from our modular structure. Can be tailored to your specific needs. Divided into 6 modules:

  • Protein Expression
  • Protein solubilization
  • Protein Stabilization
  • Protein Purification
  • Protein Characterization
  • Protein Structure Determination

If you want more information or a quote, contact us here:

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