Promos chromotek

ChromoTek Nano-Traps are the benchmark in immunoprecipitation (IP) and allow fast and reliable one-step pulldowns of even low expressed proteins. They consist of Nanobodies coupled to beads and are ready-to-use.

Profits:

• No heavy & light antibody chains
• Stringent washing conditions
• Low host cell protein background
• Very efficient pulldown, no protein in flow through
• High affinity to bind even proteins expressed at low levels
• Short incubation (30-60 min)
• Recombinantly expressed and validated

Available Nano-Trap targets:

Nano-Traps are available with up to 3 different beads:

• Agarose for very low background and high binding capacity IP
• Magnetic Agarose for magnetic separation and high binding capacity IP
• Magnetic Particles M-270 for IP of very large proteins/complexes

If you have any questions, or want to try them, ask them here:

Immunoprecipitation (IP) of a protein of interest (POI) by Nano-Traps (left) compared with conventional antibodies coupled to Protein A/G beads (right) analyzed by SDS-PAGE. When using Nano-Traps, the amount of immunoprecipitated POI is significantly higher and the background is reduced, in contrast to an IP conducted with a conventional antibody. Nano-Traps provide pure fractions of immunoprecipitated POI without contamination of heavy & light antibody chains.