Cellular heterogeneity is present in every biologic sample at gene expression or protein level. Currently, most experiments to determine comprehensive gene expression are based on bulk sample population and they are very informative. However, the analysis of bulk samples can mask the individual effects of unique cellular subsets.
With flow cytometry, million of unique cells can be detected simultaneously at membrane and intracelullar levels allowing the characterization of heterogenic populations, even though this kind of analysis can be often restricted to antibody availability.
Due to its high-throughput characterization of various cell types, flow cytometry is the gold standard for immunologists. PrimeFlow™ technology enables the detection of nucleic acid sequences at the single-cell level without being limited by antibody availability, therefore allowing the detection of many more markers that remained undetected with conventional antibody-based flow cytometry. This assay can detect a maximun of three RNAs per cell combined with the use of classic antibodies
Uses of this technique:
- Detect RNA and protein simultaneously by flow cytometry
- Compare RNA and protein kinetics in the same cell
- See gene expression heterogeneity at the single-cell level
- Detect non-coding RNA in cellular subsets
- Analyze mRNA expression levels when antibody selection is limited
- Evaluate viral RNA in infected cells
More information about PrimeFlow RNA